神经干细胞树突调节蛋白DAGLα抗体说明书

中文名称  神经干细胞树突调节蛋白DAGLα抗体       

浓    度  1mg/1ml

规 格  0.2ml/200μg          

抗体来源  Rabbit  

克隆类型  polyclonal

交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep   

产品类型  一抗    

研究领域  肿瘤 神经生物学 信号转导 干细胞  

蛋白分子量  predicted molecular weight: 115kDa

性    状  Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human DAGLA

亚    型  IgG

纯化方法  affinity purified by Protein A

储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500
（石蜡切片需做抗原修复）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

神经干细胞树突调节蛋白DAGLα抗体产品介绍 Members of the AB hydrolase superfamily have diverse catalytic functions and play a crucial role in the metabolism of lipids. DAGLAlpha (diacylglycerol lipase alpha), also known as NSDDR or C11orf11, is a 1,042 amino acid multi-pass membrane protein that belongs to the AB hydrolase superfamily. Highly expressed in brain and pancreas, DAGL?uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGLAlpha functions as at optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2. The gene encoding DAGLAlpha maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome.

Function : Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.

Subcellular Location : Cell membrane; Multi-pass membrane protein.

Tissue Specificity : Highly expressed in brain and pancreas.

DISEASE : Defects in DAGLA may be a cause of spinocerebellar ataxia type 20 (SCA20) [MIM:608687]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA20 is an autosomal dominant, adult-onset form characterized by dysarthria due to spasmodic dysphonia followed by slowly progressive ataxia. Note=A copy number variation consisting of a 260-kb duplication at chromosome 11q12.2-12.3 is responsible for SCA20. The critical gene within the duplicated segment may be DAGLA.

Similarity : Belongs to the AB hydrolase superfamily. Lipase family.

Database links : UniProtKB/Swiss-Prot: Q9Y4D2.3