磷酸酶/磷酸二酯酶家族成员6抗体应用方法

中文名称    磷酸酶/磷酸二酯酶家族成员6抗体    

英文名称    ENPP6

别    名    B830047L21Rik; E-NPP 6; Ectonucleotide pyrophosphatase/phosphodiesterase 6; ectonucleotide pyrophosphatase/phosphodiesterase family member 6; ENPP 6; NPP 6; NPP6; ENPP6_HUMAN.    

供 应 商    远慕生物    

研究领域    肿瘤  细胞生物  神经生物学  激酶和磷酸酶  新陈代谢      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 磷酸酶/磷酸二酯酶家族成员6抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    46kDa    

细胞定位    细胞膜 分泌型蛋白     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ENPP6    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸酶/磷酸二酯酶家族成员6抗体产品介绍    background:

NPP6 is a 440 amino acid member of the nucleotide pyrophosphatase/phosphodiesterase family. NPP6 is a secreted and single-pass type I membrane protein. Predominantly expressed in brain and kidney, NPP6 is a choline-specific glycerophosphodiester phosphodiesterase. NPP6 can hydrolyze the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, glycerophosphorylcholine, sphingosylphosphorylcholine and lysophosphatidylcholine (LPC). NPP6 has been found to have a preference for LPC with polyunsaturated or short fatty acids. The gene encoding NPP6 maps to human chromosome 4, which consists of approximately 6% of the human genome and nearly 900 genes. Chromosome 4 is associated with Huntington's disease, FGFR-3, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

Function:
ENPP6 is a choline-specific glycerophosphodiester phosphodiesterase, which hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5 prime-monophosphate. It hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. ENPP6 has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. ENPP6 also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. ENPP6 is predominantly expressed in kidney and brain.

Subunit:
Homodimer; disulfide-linked.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Secreted. Note: A minor secreted form also exists.

Tissue Specificity:
Predominantly expressed in kidney and brain. In the kidney, expressed specifically in the proximal tubules and thin descending limbs of Henle (at protein level).

Similarity:
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Database links:
UniProtKB/Swiss-Prot: Q6UWR7.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.