延伸因子EF-1δ抗体应用方法

中文名称    延伸因子EF-1δ抗体    

英文名称    EEF1D

别    名    Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; Eukaryotic translation elongation factor 1 delta; Guanine nucleotide exchange protein.    

供 应 商    远慕生物    

研究领域    细胞生物  转录调节因子  转运蛋白  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 延伸因子EF-1δ抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    31kDa    

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human EEF1D    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 亱C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20亱C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 亱C.    

延伸因子EF-1δ抗体产品介绍    background:

This gene encodes a subunit of the elongation factor-1complex, which is responsible for the enzymatic delivery ofaminoacyl tRNAs to the ribosome. This subunit, delta, functions asguanine nucleotide exchange factor. It is reported that followingHIV-1 infection, this subunit interacts with HIV-1 Tat. Thisinteraction results in repression of translation of host cellproteins and enhanced translation of viral proteins. Severalalternatively spliced transcript variants encoding multipleisoforms have been found for this gene. Related pseudogenes havebeen defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided byRefSeq, Aug 2010].

Function:
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

Subunit:
EF-1 is composed of 4 subunits: alpha, beta, deltaisoform 1, and gamma. Isoform 2 interacts with HSF1 and NFE2L2.

Subcellular Location:
Isoform 2: Nucleus.

Tissue Specificity:
Isoform 2 is specifically expressed in brain,cerebellum and testis.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the EF-1-beta/EF-1-delta family.

Database links:
UniProtKB/Swiss-Prot: P29692.5

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.