吞噬细胞运动蛋白2抗体应用方法

中文名称    吞噬细胞运动蛋白2抗体   

英文名称    ELMO2

别    名    CED 12; Ced 12 homolog 2; CED 12 homolog A; CED 12A; CED-12; ced-12 homolog 2; CED12; CED12 homolog A; CED12A; ELMO 2; ELMO-2; ELMO2; ELMO2_HUMAN; Engulfment and cell motility 2 (ced 12 homolog, C. elegans); Engulfment and cell motility 2; Engulfment and cell motility protein 2; FLJ11656; hCED 12A; hCED-12A; hCED12A; KIAA1834; PH domain protein CED12A; Protein ced 12 homolog A; Protein ced-12 homolog A.   

供 应 商    远慕生物 

研究领域    细胞生物  信号转导  细胞凋亡     

抗体来源    Rabbit   

克隆类型    Polyclonal   

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,    

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 吞噬细胞运动蛋白2抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.   

分 子 量    83kDa   

细胞定位    细胞浆 细胞膜    

性    状    Lyophilized or Liquid   

浓    度    1mg/1ml   

免 疫 原    KLH conjugated synthetic peptide derived from human ELMO2   

亚    型    IgG   

纯化方法    affinity purified by Protein A   

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4   

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.   

吞噬细胞运动蛋白2抗体产品介绍    background:

Elmo (engulfment and cell motility) proteins share similarity to C. elegans CED-12. The C. elegans genes ced-2, ced-5, ced-10, and ced-12 and their mammalian homologs CRKII, DOCK1, RAC1 and ELMO mediate cytoskeletal rearrangements during phagocytosis of apoptotic cells and cell motility. Elmo1 associates with DOCK 180 and may influence phagocytosis and effect cell shape changes. Src family kinase mediated tyrosine phosphorylation of ELMO1 influences signaling through Elmo1/Crk/DOCK 180 pathways. Elmo2 interacts directly with Rho G in a GTP-dependent manner and forms a ternary complex with DOCK 180 to induce activation of Rac 1. The RhoG-Elmo2-DOCK 180 pathway is required for activation of Rac 1 and cell spreading mediated by integrin, as well as for neurite outgrowth induced by nerve growth factor. Elmo3 acts in assocation with DOCK180 and Crk II and may be required in complex with DOCK180 to activate Rac/Rho small GTPases.

Function:
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

Subunit:
Interacts with the SH3-domain of DOCK1 via its SH3-binding site. Probably part of a complex with DOCK1 and RAC1. Probably part of a complex with DOCK1 and CRK isoform CRK-II. Interacts with ARHGEF16, DOCK4 and EPHA2; mediates activation of RAC1 by EPHA2.

Subcellular Location:
Cytoplasm. Cytoplasm; cytosol. Membrane.

Tissue Specificity:
Widely expressed, with a higher expression in skeletal muscle, kidney and placenta.

Similarity:
Contains 1 ELMO domain.
Contains 1 PH domain.

Database links:
UniProtKB/Swiss-Prot: Q96JJ3.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.