磷酸化DDX58抗体产品应用说明

中文名称    磷酸化DDX58抗体   

英文名称    phospho-DDX58 (Ser8) 

别    名    p-DDX58 (Ser8); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I; DKFZp434J1111; DKFZp686N19181; FLJ13599; C330021E21; OTTHUMP00000045225; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD (Asp Glu Ala Asp/His) box polypeptide; DEAD box protein 58; Probable ATP dependent RNA helicase DDX58; Retinoic acid inducible gene 1 protein; RIG I; rig-I; RIG1; rigi; RNA helicase.   

供 应 商    远慕生物     

产品类型    磷酸化抗体    

研究领域    肿瘤  免疫学  信号转导  细胞凋亡  细胞分化     

抗体来源    Rabbit   

克隆类型    Polyclonal   

交叉反应    Human,    

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500磷酸化DDX58抗体 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.   

分 子 量    102kDa   

细胞定位    细胞浆    

性    状    Lyophilized or Liquid   

浓    度    1mg/1ml   

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human DDX58 around the phosphorylation site of Ser8   

亚    型    IgG   

纯化方法    affinity purified by Protein A   

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide   

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.   

磷酸化DDX58抗体产品介绍    background:

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG1/DDX58) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG1/DDX58 activates the kinases TBK1 and IKKe through the adaptor protein IPS1. These kinases then phosphorylate the transcription factors IRF3 and IRF7 which are essential for the expression of type-I interferons. RIG1/DDX58 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

Function:
Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.

Subunit:
Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58. Interacts with SRC.

Subcellular Location:
Cytoplasm. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies.

Tissue Specificity:
Present in vascular smooth cells (at protein level).

Post-translational modifications:
Phosphorylated in resting cells and dephosphorylated in RNA virus-infected cells. Phosphorylation at Thr-770, Ser-854 and Ser-855 results in inhibition of its activity while dephosphorylation at these sites results in its activation.
Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
Ubiquitinated. Undergoes 'Lys-48'- and 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS/IPS1 binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. Also probably deubiquitinated by USP17L2/USP17 that cleaves 'Lys-48'-and 'Lys-63'-linked ubiquitin chains and positively regulates the receptor.