DENND1B蛋白抗体说明书

英文名称    DENND1B   
中文名称    DENND1B蛋白抗体   
别    名    C1ORF18; C1orf218; Chromosome 1 open reading frame 218; Connecdenn 2; DEN1B_HUMAN; DENN domain containing protein 1B; DENN domain-containing protein 1B; DENN MADD domain containing 1B; DENND1B; DKFZp547O0715; FAM31B; Family with sequence similarity 31 member B; FLJ20054; FLJ21179; FLJ33888; MGC103810; MGC27044; Protein FAM31B   
说 明 书    0.2ml     
研究领域    细胞生物  免疫学     
抗体来源    Rabbit   
克隆类型    Polyclonal   
交叉反应    Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,    
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 DENND1B蛋白抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.   
分 子 量    48kDa   
细胞定位    细胞浆    
性    状    Lyophilized or Liquid   
浓    度    1mg/1ml   
免 疫 原    KLH conjugated synthetic peptide derived from human DENND1B   
亚    型    IgG   
纯化方法    affinity purified by Protein A   
储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4   
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.  
 
DENND1B蛋白抗体产品介绍    background:
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.

Tissue specificity:Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.

Function:
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactivates RAB35, then activates RAB35 for its subsequent function at endosomes.

Subunit:
Interacts with RAB35 in the absence of nucleotide, but not in the presence of GTP. Interacts with clathrin heavy chain/CLTC and with AP2A2. Isoform 5 interacts with AP2B1.

Subcellular Location:
Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle.

Tissue Specificity:
Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells.

Post-translational modifications:
Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.