有丝分裂控制蛋白dis3抗体实验用途

英文名称  Anti-DIS3 
中文名称  有丝分裂控制蛋白dis3抗体 
别    名  Dis3; DIS3 mitotic control homolog (S. cerevisiae); dis3p; Exosome complex exonuclease RRP44; Mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA processing protein 44; Ribosomal RNA-processing protein 44; RRP44; RRP44_HUMAN. 
应用于医学免疫学、动物免疫学、分子生物学、生物化学、临床医学、检验医学、动物医学、药学、理工、农学、环境学等生命科学密切相关的专业。          
浓    度  1mg/1ml 
规 格  0.2ml/200μg          
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat, Dog, Horse, Rabbit
产品类型  一抗    
研究领域  细胞生物 细胞周期蛋白 细胞分化 表观遗传学  
蛋白分子量  predicted molecular weight: 105kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from human DIS3 (701-730aa) 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

有丝分裂控制蛋白dis3抗体产品介绍 Component of the exosome that has both 3'-5' exonuclease and endonuclease activities. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. As part of nucleoplasmic and probably cytoplasmic forms of the RNA exosome complex, it is involved in RNA processing and turnover. Also associated with the GTPase RAN.
Function : Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Subunit : Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic-and nuclear-specific RNA exosome complex forms.
Subcellular Location : Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Note=Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
Tissue Specificity : Widely expressed.
Similarity : Belongs to the RNR ribonuclease family.
Contains 1 PINc domain.

【存储要求】连续使用时4°C存储，保质期六个月；有丝分裂控制蛋白dis3抗体期存储时建议分装为10ul以上小包装-20°C存储，并避免反复冻融，保质期一年。

实验的用途：
1）WB：Western Blotting 免疫印迹
2）IH：Immunohistochemistry 免疫组化
3）IH(P)：Immunohistochemistry Parraffin sections 免疫组化(石蜡)
4）IH(F)：Immunohistochemistry Frozen sections 免疫组化(冰冻)
5）IC：Immunocytochemistry 免疫细胞化学