视神经细胞相关蛋白/早老素结合蛋白抗体应用说明

产品名称：视神经细胞相关蛋白/早老素结合蛋白抗体
别名：Dedicator of cytokinesis protein 3; DOCK 3; MOCA; Modifier of cell adhesion; Presenilin binding protein; DOCK3_HUMAN.
浓度：1mg/1ml
规格：0.2ml/200μg          
抗体来源：Rabbit  
克隆类型：polyclonal
交叉反应：Human, Mouse, Rat, Dog, Pig, Rabbit   
产品类型：视神经细胞相关蛋白/早老素结合蛋白抗体一抗    
研究领域：神经生物学 信号转导 干细胞 Alzheimer's  
蛋白分子量：predicted molecular weight: 233kDa
性状：Lyophilized or Liquid
免疫原：KLH conjugated synthetic peptide derived from human DOCK3 (1001-1100aa)
亚型：IgG
纯化方法：affinity purified by Protein A
储存液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用：WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500
（石蜡切片需做抗原修复）
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍：MOCA (modifier of cell adhesion), also known as Presenilin-binding protein (PBP) or dedicator of cytokinesis protein 3 (DOCK3), is a 2030 amino acid cytoplasmic protein belonging to the DOCK family. MOCA interacts with Presenilin proteins and has the ability to stimulate Tau phosphorylation suggesting that MOCA may be involved in Alzheimer disease. MOCA is also thought to be a guanine nucleotide exchange factor (GEF) which activates small GTPases by exchanging bound GDP for free GTP. Analysis of ectopic expression suggests that MOCA may affect the function of small GTPases involved in the regulation of Actin cytoskeleton or cell adhesion receptors. MOCA is localized to the neuropil, and sometimes in pyramidal cells, in normal brains, while in Alzheimer disease brains, MOCA is present in neurofibrillary tangles.

Function : DOCK3 is a potential Guanine Nucleotide Exchange factor, present predominantly in brain. DOCK3 is localised in the neuropil and occasionally in the pyramidal cells. In brains of patients with Alzheimer's disease, it is associated with neurofibrillary tangles. DOCK3 may be required for maintaining the functional integrity of axons and loss of DOCK3 may result in axonal degeneration. It is associated with behavioural/developmental conditions like Attention Deficit Hyperactivity disorder.

Subunit : Interacts with presenilin proteins PSEN1 and PSEN2. Interacts with CRK

Subcellular Location : Cytoplasmic

Tissue Specificity : In normal brains, it is localized in the neuropil, and occasionally in the pyramidal cells, while in Alzheimer disease brains, it is associated with neurofibrillary tangles. [DOMAIN] The DHR-2 domain may mediate some GEF activity

DISEASE : Note=A chromosomal aberration involving DOCK3 has been found in a family with early-onset behavioral/developmental disorder with features of attention deficit-hyperactivity disorder and intellectual disability. Inversion inv(3)(p14:q21). The inversion disrupts DOCK3 and SLC9A9.

Similarity : Belongs to the DOCK family.

Contains 1 DHR-1 domain.

Contains 1 DHR-2 domain.

Contains 1 SH3 domain.

Database links : UniProtKB/Swiss-Prot: Q8IZD9.1